Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression. Escherichia coli BL21(DE3)

We have previously reported that phosphoenolpyruvate carboxykinase(Pck) overexpression under glycolytic conditions enables Escherichia coli to harbor a high intracellular ATP pool resulting in enhanced recombinant protein synthesis and biohydrogen production. To understand possible reasons of the high ATP haboring cell, we carried out transcriptome and metabolic flux analysis. Overall design: Comparison of main metabolic gene and regulator expression profiling using RNA-seq data of BL21(DE3) vs. BL21(DE3)_Pck_over under glucose LB

本团队此前已有研究报道：在糖酵解条件下过表达磷酸烯醇式丙酮酸羧激酶（phosphoenolpyruvate carboxykinase, Pck）可使大肠杆菌（Escherichia coli）拥有较高的胞内ATP池，进而提升重组蛋白合成效率与生物制氢能力。为探究该高ATP含量菌株的潜在成因，本研究开展了转录组学与代谢流分析。实验总体设计：以葡萄糖LB培养基培养的BL21(DE3)菌株与过表达Pck的BL21(DE3)菌株为研究对象，通过RNA测序（RNA-seq）分析二者的主要代谢基因及调控因子表达谱差异。