Comparing the microbiota composition between HPV positive and negative patients with CRC. Comparing the microbiota composition between HPV positive and negative patients with CRC

Total DNA was extracted from the stool of the patients, amplified to collect amplicons of variable V3–V4 regions (primers 341F and 805R) of the bacterial 16s rRNA gene and sequenced with MiSeq (2x300bp) Illumina platform. Overall design: Total DNA was extracted from the stool of the patients, amplified to collect amplicons of variable V3–V4 regions (primers 341F and 805R) of the bacterial 16s rRNA gene and sequenced with MiSeq (2x300bp) Illumina platform.

从患者粪便中提取总DNA，扩增获取细菌16S rRNA基因的V3–V4可变区扩增子（所用引物为341F与805R），随后采用Illumina MiSeq（2×300bp）测序平台完成测序。整体实验设计：从患者粪便中提取总DNA，扩增获取细菌16S rRNA基因的V3–V4可变区扩增子（所用引物为341F与805R），随后采用Illumina MiSeq（2×300bp）测序平台完成测序。