GSE47264: CTCF.14-16 hr OR embryo.Solexa

Summary: modENCODE_submission_5069 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 14-16hr OR; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody CTCF (target is CTCF); Developmental Stage Embryo 14-16hr OR

摘要：modENCODE_submission_5069 本次提交来自Gary Karpen主导的modENCODE项目。如需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。项目目标：本研究旨在确定125种染色体蛋白在黑腹果蝇（Drosophila melanogaster）基因组中的定位。所研究的蛋白参与DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。本研究将依托Illumina下一代测序（Next-Generation Sequencing, NGS）平台开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。初期将采用三种细胞系与两个胚胎发育阶段的染色质开展定位分析，后续将把部分蛋白的分析范围拓展至另外四种动物组织/发育阶段。关于数据使用的条款与细则，请参阅http://www.genome.gov/27528022 及 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。总体实验设计：实验类型：染色质免疫沉淀测序（ChIP-seq）。生物样本来源：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；发育阶段：胚胎14-16小时（Oregon-R品系）；基因型：野生型；实验因素：品系：Oregon-R（官方命名：Oregon-R-modENCODE，基因型：野生型）；抗体：CTCF（靶标为CTCF蛋白）；发育阶段：胚胎14-16小时（Oregon-R品系）