Table_1_CircPIK3C2A Facilitates the Progression of Glioblastoma via Targeting miR-877-5p/FOXM1 Axis.xls

Glioblastoma is a rare yet lethal type of tumor that poses a crucible for the medical profession, owing to its rapid proliferation and invasion resulting in poor prognosis. Circular RNAs (circRNAs), a subclass of regulatory RNAs, are implicated in the regulation of cancerous progression. This study aims to investigate the roles and underlying mechanism of circPIK3C2A in regulating proliferation and invasion of glioblastoma. qRT-PCR assays showed that the expression level of circPIK3C2A was aberrantly higher in glioblastoma cell lines, in comparison with that in normal glia cells. The ectopic expression of circPIK3C2A promoted the proliferation, invasion and clonal formation of glioblastoma cells, while circPIK3C2A loss-of-function exerted exactly the opposite biological effects on the cells. The construction of subcutaneous xenograft tumor model in nude mice indicated that circPIK3C2A loss-of-function effectively diminished tumor load in vivo and prolonged the survival time of tumor-bearing animals. Luciferase reporter assay confirmed the interaction among circPIK3C2A/miR-877-5p and FOXM1. CircPIK3C2A function as competitive endogenous RNA via sponging miR-877-5p through certain binding sites, thereby modulating the expression of FOXM1. Our results collectively indicate that circPIK3C2A functions as ceRNA by mediating miR-877-5p/FOXM1 axis, providing a novel perspective of applying CircPIK3C2A in the clinical intervention of glioblastoma in the future.

胶质母细胞瘤（Glioblastoma）是一种罕见却具有致死性的肿瘤，因其快速增殖与侵袭特性导致预后不良，始终是医学界面临的严峻挑战。环状RNA（circRNAs）作为调控RNA的一个子类，已被证实参与癌症进展的调控过程。本研究旨在探讨circPIK3C2A在胶质母细胞瘤增殖与侵袭中的作用及潜在分子机制。实时荧光定量PCR（qRT-PCR）检测结果显示，与正常胶质细胞相比，circPIK3C2A在胶质母细胞瘤细胞系中呈异常高表达。异位表达circPIK3C2A可促进胶质母细胞瘤细胞的增殖、侵袭与克隆形成能力，而circPIK3C2A功能缺失则对细胞产生完全相反的生物学效应。裸鼠皮下异种移植瘤模型实验表明，circPIK3C2A功能缺失可有效降低体内肿瘤负荷，并延长荷瘤小鼠的存活时间。荧光素酶报告基因实验证实，circPIK3C2A、miR-877-5p与FOXM1三者间存在相互作用。circPIK3C2A通过特定结合位点吸附miR-877-5p，发挥竞争性内源RNA（ceRNA）的作用，进而调控FOXM1的表达。本研究结果综合表明，circPIK3C2A通过介导miR-877-5p/FOXM1轴发挥ceRNA功能，为未来胶质母细胞瘤的临床干预提供了全新的研究视角。