Haplotypic polymorphisms and forensic applications in Chinese Hunan Han population based on a series of Y-STR loci: a perspective of paternal inheritance

Hunan, a multinational province in China, possesses more than fifty ethnic groups, such as the Han, Yao, Tujia, Miao, and so on. To evaluate the forensic efficiency of the novel panel and investigate the genetic relationships between Hunan Han population and 12 other reference populations from China. Haplotypic data of 153 unrelated males of Hunan Han population were investigated using the AGCU Y SUPP Plus amplification system containing 27 Y-chromosomal short tandem repeat (STR) loci. Forensic parameters were calculated to evaluate the application efficiency of this panel in Hunan Han population. Haplotype diversity, discrimination capability, and match probability values were 0.9999999977, 1.0000, and 0.0065, respectively. Pairwise fixation index values demonstrated that the minimal genetic differentiation (0.0073) was found between Hunan Han population and Hunan Yao group, while the maximal genetic differentiation (0.0651) was observed between Hunan Han and Guangxi Yao group from the perspective of the patrilineal DNA analysis. The haplotype distributions of 27 Y-STR loci in Hunan Han population exhibited remarkable polymorphisms. Moreover, this panel has potential advantages for the forensic applications regarding family investigations, paternity testing of the paternal line, and population genetics.

中国多民族省份湖南聚居着汉族、瑶族、土家族、苗族等五十余个族群。本研究旨在评估该新型扩增体系的法医学效能，并探究湖南汉族群体与国内12个参考群体之间的遗传亲缘关系。研究采用包含27个Y染色体短串联重复序列（short tandem repeat, STR）位点的AGCU Y SUPP Plus扩增体系，对153名无亲缘关系的湖南汉族男性的单倍型数据进行检测分析。通过计算相关法医学参数，评估该体系在湖南汉族群体中的应用效能。结果显示，单倍型多样性、个体识别能力与匹配概率分别为0.9999999977、1.0000和0.0065。基于父系DNA分析的两两群体固定指数结果表明，湖南汉族群体与湖南瑶族群体之间的遗传分化程度最低（0.0073），而湖南汉族群体与广西瑶族群体之间的遗传分化程度最高（0.0651）。湖南汉族群体27个Y-STR位点的单倍型分布呈现出显著的多态性。此外，该扩增体系在家族溯源调查、父系亲子鉴定以及群体遗传学研究等法医学应用场景中具备潜在应用优势。