Gene expression in cauliflower curds. Gene expression in cauliflower curds

Description of gene expression in various types of cauliflower (including Romanesco type) Cauliflower structures develop on inflorescences from plants such as the apetala1 cauliflower double mutant in Arabidopsis thaliana or in the edible Brassica oleracea var botrytis or Romanesco. Whereas the genetic basis for the development of such structure is well understood in the model plant Arabidopsis thaliana, it is less understood in Brassica oleracea. In order to gain insight into the gene expression present in cauliflower and Romanesco curds, we performed RNA-seq analyses of mature cauliflowers and Romanesco curds and from growing cauliflowers curds. This analysis allowed to establish the nature of genes from the APETALA1 and CAULIFLOWER clade that are either mutated or expressed at very low levels in curd structures. These results shed light on the molecular nature of structures that have been selected in the domestication process. Overall design: Cauliflower were harvested from field experiments or bought at producer and RNA-seq was performed on curd sample

本数据集描述了不同类型花椰菜（cauliflower，包括罗马花椰菜（Romanesco）类型）的基因表达特征。花椰菜结构可在花序上发育，其来源包括拟南芥（Arabidopsis thaliana）的apetala1与cauliflower双突变体，或是可食用的芸薹属甘蓝花椰菜变种（Brassica oleracea var. botrytis）以及罗马花椰菜。尽管这类结构发育的遗传基础在模式植物拟南芥中已得到充分阐明，但在芸薹属甘蓝（Brassica oleracea）中相关机制仍不甚明确。为深入解析花椰菜与罗马花椰菜花球中的基因表达谱，本研究对成熟花椰菜花球、罗马花椰菜花球以及发育中的花椰菜花球开展了RNA测序（RNA-seq）分析。通过该分析，本研究明确了APETALA1（APETALA1）与CAULIFLOWER（CAULIFLOWER）进化支基因在花球结构中发生突变或呈低水平表达的特征。上述研究结果揭示了驯化过程中被选择的花椰菜结构的分子本质。实验设计：花椰菜材料分别采集自田间试验或从生产商处购得，本研究对其花球样本进行了RNA-seq测序分析。