ChIP-Seq analysis of Myc-tagged budding yeast Rif1. ChIP-Seq analysis of Myc-tagged budding yeast Rif1

Genomic locations of Myc-tagged budding yeast Rif1 (including wilt-type and designer mutants) were analysed by ChIP-Seq. Mutants tested were rif1-7A and rif1-7E, in which Ser/Thr residues in the cluster of SQ/TQ sites were mutated to Ala or Glu, respectively. The performance of RIF1-9V5 was also tested.

本研究通过染色质免疫共沉淀测序（ChIP-Seq）技术，分析了带Myc标签的出芽酵母Rif1（包括野生型（wild-type）与人工设计构建的突变体）的基因组定位位点。本次测试的突变体包括rif1-7A与rif1-7E，二者SQ/TQ位点簇内的丝氨酸/苏氨酸残基分别被突变为丙氨酸（Ala）或谷氨酸（Glu）。此外，本研究还对RIF1-9V5的相关实验性能进行了检测。