Expression data of differentiated human erythroid cells with or without Tranylcypromine (TC) treatment

We found that LSD1 inhibition by a monoamine oxidase inhibitor, tranylcypromine (TC), could enhance fetal gamma globin expression. Global effects of TC on erythroid expession were conducted by HG-U219 array strips. Primary human erythroid cells, which differentiated from CD34+ cells for 8 days, were harvested before or and after TC treatment (0.5 µM, 1.5 µM or 5 µM) for the gene expression analysis.

我们发现，采用单胺氧化酶抑制剂（monoamine oxidase inhibitor）反苯环丙胺（tranylcypromine，TC）抑制赖氨酸特异性去甲基化酶1（LSD1），可增强胎儿γ珠蛋白（fetal gamma globin）的表达。为分析TC对红细胞系基因表达的全局影响，本研究通过HG-U219基因芯片条带开展相关实验。实验所用的原代人红细胞系细胞由CD34阳性细胞（CD34+）诱导分化8天后获得，分别在经0.5 µM、1.5 µM或5 µM浓度的TC处理前后收集样本，用于后续基因表达分析。