Populus nigra Genotype-x-Drought, UGOE dataset. Populus nigra

This study, as part of the GxD experiment of T1.1 in the project WATBIO, investigates 3 Populus nigra genotypes at different levels of drought stress under tightly controlled environmental conditions. Non-destructive measurements of photosynthesis, water use, and water status will be conducted in addition to NMR for water flux and innovative volatile measurements and leaf turgor. Leaves, developing xylem, and roots will be harvested for transcriptome (RNA-Seq), phosphoproteome and metabolome analyses, as well as for analyses of secondary metabolites, LOX, oxidative stress markers and phytohormones. Carbohydrates, carbon discrimination (13C) and amino acids will also be determined. Wood properties (anatomy, chemical composition, saccharification) will be measured. This data set comprises RNAseq data from leafs and fine roots of 12 week old plants from three different ecotypes after 5 weeks of drought treatment and control conditions.

本研究作为WATBIO项目T1.1任务中GxD实验的组成部分，在严格可控的环境条件下，针对3个欧洲黑杨（Populus nigra）基因型开展不同程度干旱胁迫相关实验。实验将开展光合作用、水分利用与水分状态的无损检测，辅以用于水分通量分析的核磁共振（NMR）、创新性挥发性物质测定及叶片膨压测量。研究人员将采集叶片、发育中木质部与根系样本，用于转录组（RNA-Seq）、磷酸化蛋白质组与代谢组分析，同时开展次生代谢物、脂氧合酶（LOX）、氧化应激标志物及植物激素的相关检测；此外还将测定碳水化合物、碳同位素判别值（¹³C）与氨基酸含量。木材特性（包括解剖结构、化学成分与糖化效率）也将被测定。本数据集涵盖经5周干旱处理及对照条件培育的12周龄植株的叶片与细根的RNA测序数据，涉及3个不同生态型的材料。