Unconventional ST2- and CD127-negative lung ILC2 populations are induced by the fungal allergen Alternaria

Group 2 innate lymphoid cells (ILC2s) have been recognized for their ability to drive type 2 responses in experimental animal models in the absence of T cells. Mice intranasally challenged with Alternaria alternata, a fungal allergen associated with severe asthma and fatal exacerbations in humans, potently activates ILC2s via the IL-33/ST2 (IL-33R) axis to robustly secrete type 2 cytokines. Animal models, including Alternaria-induced lung inflammation in mice, have given us critical insight into pathways of ILC2 regulation that appear to apply to ILC2 responses in human disease.7 Thus, accurate identification of ILC2s in the lungs of mice is critical to future understanding of their biology and contribution to allergic disease. We assessed whether the conventional ILC2 identification markers ST2 and CD127.

2型先天淋巴细胞（Group 2 innate lymphoid cells, ILC2s）已被证实可在缺乏T细胞的实验动物模型中介导2型免疫应答。将与人类重症哮喘及致命性急性加重相关的真菌变应原链格孢菌（Alternaria alternata）经鼻内途径感染小鼠，可通过IL-33/ST2（IL-33受体）轴强效激活ILC2，使其大量分泌2型细胞因子。包括小鼠链格孢菌诱导性肺部炎症在内的动物模型，为我们理解ILC2调控通路提供了关键见解，而这些通路似乎也适用于人类疾病中的ILC2应答[7]。因此，精准鉴定小鼠肺部的ILC2，对于未来阐明其生物学特性及其在过敏性疾病中的作用至关重要。我们评估了常规ILC2鉴定标志物ST2与CD127。