Cell host-response to infection with novel human coronavirus EMC predict potential antivirals and important differences with SARS-coronavirus.

Differential expression was determined in Calu-3 cells between mock infected and infection with either Human coronavirus EMC and SARS coronavirus at different times post infection. Calu-3 2B4 cells were infected with Human Coronavirus EMC 2012 (HCoV-EMC) or mock infected. Samples were collected 0, 3, 7, 12, 18 and 24 hpi. There are 3 mock and 3 infected replicates for each time point, except for 12 hpi for which there are only 2 infected replicates (one replicate did not pass RNA quality check). There were no mock sampes at 18 hpi, and therefore infected samples at 18 hpi were compared with mocks at 24 hpi. For direct comparison with SARS-CoV infected cells, raw data from HCoV-EMC experiments were quantile normalized together with the SARS-CoV dataset (GEO Series accession number GSE33267).本研究旨在分析Calu-3细胞在假感染、感染人类冠状病毒EMC或严重急性呼吸综合征冠状病毒后的不同感染后时间点的差异表达情况。实验采用Calu-3 2B4细胞，以2012年人类冠状病毒EMC（Human Coronavirus EMC 2012，HCoV-EMC）进行感染或实施假感染处理，并于感染后0、3、7、12、18及24小时（hours post infection，hpi）采集样本。每个时间点均设置3个假感染生物学重复样本与3个感染生物学重复样本，但12 hpi时间点仅留存2个感染重复样本（其中1个重复样本未通过RNA质量检测）。18 hpi时间点未设置假感染样本，因此该时间点的感染样本需与24 hpi时间点的假感染样本进行对比分析。为与SARS冠状病毒（SARS-CoV）感染细胞的实验开展直接对照，本研究将HCoV-EMC实验的原始数据与SARS-CoV数据集（基因表达汇编（Gene Expression Omnibus，GEO）系列登录号GSE33267）进行合并分位数归一化处理。