We used comparative transcriptomics to reveal genes that are affected by cln5-deficiency and make connections to aberrant phenotypes we identified in our previous paper. An altered transcriptome underlies cln5-deficiency phenotypes in Dictyostelium discoideum

We used comparative transcriptomics to reveal genes affected by cln5-deficiency and make associations to aberrant phenotypes previously identified in cln5- Dictyostelium cells. We follow up with western blotting to see the effect at a protein level.

本研究采用比较转录组学（comparative transcriptomics）技术，揭示受cln5缺陷影响的基因，并将这些基因与此前在cln5缺陷型盘基网柄菌细胞中鉴定到的异常表型建立关联。随后通过蛋白质印迹法（western blotting）在蛋白质水平上观测其效应。