BAFF engages distinct NFkappaB effectors in maturing and proliferating B cells. Mus musculus

Identify genes which are induced in wild type B cells under antigenic stimuli, anti-IgM vs pro-survival stimuli, BAFF. Also find the differential expressed genes which are distinct from in co-stimulated conditions. Overall design: RNAseq analysis of wild type primary B cells stimulated with anti-IgM alone, BAFF alone, or co-stimulated with both anti-IgM and BAFF for 8 and 30 hours

本数据集旨在开展以下两项研究：一是鉴定野生型B细胞（wild type B cells）在抗原刺激物抗IgM（anti-IgM）与促存活刺激物BAFF单独处理下被诱导表达的基因；二是筛选出与抗IgM与BAFF共刺激条件下表达谱存在显著差异的独特基因。实验整体设计：对野生型原代B细胞（primary B cells）分别施以单独抗IgM刺激、单独BAFF刺激，以及抗IgM与BAFF联合共刺激三种处理，分别于刺激8小时和30小时后采集样本，进行RNA测序（RNAseq）分析。