Effect of developmental (24-100 hpf) embryonic exposures to tris(4-chlorophenyl)methane and tris(4-chlorophenyl)methanol in the zebrafish

Tris(4-chlorophenyl)methane (TCPM) and tris(4-chlorophenyl)methanol (TCPMOH) are two marine pollutants of emerging concern, found in marine mammals and coastal bird populations. Here, we assess the response to these compounds due to developmental (24-100 hourrs post fertilization) exposures in zebrafish embryos Overall design: We analyzed a total of 15 samples: Samples 1-5 were controls, exposed to 0.01% v/v dimethyl sulfoxide for 3 days between 24-100 hours post fertilization. Samples 6-10 were exposed to 50 nM TCPM, and Samples 11-15 were exposed to 50 nM TCPMOH. Samples were collected from 3 experimental replicates, with randomized embryos acquired from 20 breeding pairs.

三(4-氯苯基)甲烷（Tris(4-chlorophenyl)methane, TCPM）与三(4-氯苯基)甲醇（Tris(4-chlorophenyl)methanol, TCPMOH）是两类备受关注的新型海洋污染物，已在海洋哺乳动物及海岸鸟类种群中被检出。本研究针对斑马鱼胚胎在受精后24至100小时的发育阶段暴露于这两种化合物后的响应展开评估。实验设计：本研究共分析15份样本，其中样本1至5为对照组，于受精后24至100小时期间以0.01%体积比的二甲基亚砜暴露3天；样本6至10暴露于50纳摩尔的TCPM，样本11至15则暴露于50纳摩尔的TCPMOH。所有样本均设置3次生物学重复，实验所用胚胎均取自20对繁育亲鱼的随机胚胎。