ion intensity (merged)

Intra- and extra- cellular non-targeted metabolomes of primary human monocyte-derived macrophages. Table contains a list of ions, m/z ratio, putative annotation based on m/z ratio, module membership and log intensities.
Experimental protocol: PBMCs were isolated from leukoreduction system chambers and buffy coats obtained with written informed consent from healthy anonymous blood donors in the UK and The Netherlands, respectively. CD14+ monocytes were isolated using CD14 magnetic beads and MACS columns (Miltenyi). The subsequent monocytes were then differentiated into macrophages over 6 days using 50 ng/mL human M-CSF (Miltenyi) and cultured in Iscove's Modified Dulbecco's Medium (IMDM) supplemented with 10% heat-inactivated fetal bovine serum, 1% Penicillin/Streptomycin solution, and 1% L-glutamine solution (Gibco). On day 6, the medium was replaced with culture medium without M-CSF and supplemented with the following stimuli: no stimulus, 10 ng/mL LPS (Sigma, E. coli E55:O5), 10 ng/mL LPS plus 50 ng/mL IFN-γ (R&D), 50ng/mL IFN-γ (R&D), 50 ng/mL IL-4 (PeproTech), 50 ng/mL IL-10 (R&D), 100 nM dexamethasone (Sigma) for 24 hours.

Annotation:
M0: unstimulated macrophages (24hr medium)
M-LPS: 24hr LPS,
M-LPSIFNγ: 24hr LPS and IFN-γ,
M-IFNγ: 24hr IFN-γ,
M-IL4: 24hr IL-4,
M-IL10: 24hr IL-10,
M-dex: 24hr dexamethasone
numbers at the end of the sample ID represent donor numbers.
associated preprint: https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4647290

原代人单核细胞源性巨噬细胞的胞内与胞外非靶向代谢组。本数据集表格包含离子列表、质荷比、基于质荷比的推定注释、模块归属以及对数强度值。

实验方案：分别从英国与荷兰的健康匿名献血者签署书面知情同意书后获取的白细胞滤除系统容器及血沉棕黄层中分离外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）。使用CD14磁珠与磁性活化细胞分选柱（Magnetic Activated Cell Sorting, MACS，Miltenyi）分离CD14阳性单核细胞；随后将单核细胞置于添加了50 ng/mL人巨噬细胞集落刺激因子（Macrophage Colony-Stimulating Factor, M-CSF，Miltenyi）的培养基中培养6天，诱导分化为巨噬细胞，所用培养基为伊斯科夫改良杜尔贝科培养基（Iscove's Modified Dulbecco's Medium, IMDM），并补充10%热灭活胎牛血清、1%青霉素-链霉素溶液及1% L-谷氨酰胺溶液（Gibco）。于培养第6天，更换不含M-CSF的培养基，并添加以下刺激物继续培养24小时：无刺激、10 ng/mL脂多糖（Lipopolysaccharide, LPS，Sigma，大肠杆菌E55:O5）、10 ng/mL LPS联合50 ng/mL干扰素-γ（Interferon-γ, IFN-γ，R&D）、50 ng/mL IFN-γ（R&D）、50 ng/mL白细胞介素-4（Interleukin-4, IL-4，PeproTech）、50 ng/mL白细胞介素-10（Interleukin-10, IL-10，R&D）及100 nM地塞米松（Sigma）。

注释：
M0：未受刺激的巨噬细胞（24小时培养基培养）
M-LPS：24小时脂多糖刺激组
M-LPSIFNγ：24小时脂多糖联合干扰素-γ刺激组
M-IFNγ：24小时干扰素-γ刺激组
M-IL4：24小时白细胞介素-4刺激组
M-IL10：24小时白细胞介素-10刺激组
M-dex：24小时地塞米松刺激组
样本ID末尾的数字代表供者编号。
相关预印本：https://papers.ssrn.com/sol3/papers.cfm?abstract_id=4647290