Human placental tissue LC-MS/MS

This project aimed at missing protein identification from human placental tissue, combining sample preparation and mass spectrometry technology development.For sample preparation, low molecular proteins were enriched by C18 solid phase extraction, SDS-PAGE and MWCO filter. For LC-MS/MS analysis, micro-HPLC fractionation was carried out coupled with LUMOS instrument. Proteome Discoverer 2.2 software was used for data analysis to identify missing proteins.

本项目针对人类胎盘组织的缺失蛋白质鉴定展开研究，整合了样品前处理与质谱技术开发工作。样品前处理环节采用C18固相萃取、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）以及分子量截留（MWCO）滤膜对低分子蛋白质进行富集。液相色谱-串联质谱（LC-MS/MS）分析阶段，通过微型高效液相色谱（micro-HPLC）进行分级分离，并联用LUMOS质谱仪完成检测。数据分析工作依托Proteome Discoverer 2.2软件开展，以实现缺失蛋白质的鉴定。