Purification and In Vitro and In Vivo Expression Mechanism of Anti-Tumor Substances from Amphibians

This research seeks to investigate the potential effects of certain biological compounds obtained from amphibian sources on tumor development and their underlying processes. By analyzing tumor size, mass, proliferation rate, apoptosis levels, and gene expression markers, cell death rates, as well as changes in genetic markers, these findings suggest promising therapeutic potential, warranting further preclinical validation. Statistical methods were applied to assess possible applications in cancer treatment strategies. The test period was from the beginning of 2024 to mid-May of 2025, and the test group consisted of 60 amphibians, thirty of which were given the amphibian compounds, and thirty of which were treated with a saline solution. Data were analyzed using the SPSS program, version 26.0, using t-tests and chi-squared tests. The P value, below 0.05, was considered statistically significant. During the twelve-week observation period, the dimensions and masses of the tumors were regularly measured and recorded. In addition, the cell proliferation, apoptosis, and activity of certain genetic factors were also measured. A decrease in the tumor size, mass, and cell division rate compared to the control group (P=0.0005). The group of treated animals showed a significant increase in cell death (P=0.0001) and a change in genetic markers (P=0.0001). As a possible therapeutic agent, this material gives us some support for the possibility of new approaches to cancer treatment, but the results require further verification to confirm practical application. The study has revealed that amphibian-derived compounds can reduce the size of tumors, alter cell growth, promote apoptosis, and influence gene expression, all of which suggest the anti-cancer properties of this material.

本研究旨在探究从两栖动物中提取的特定生物活性化合物对肿瘤发生发展及其潜在分子机制的影响。本研究通过检测肿瘤体积、瘤重、细胞增殖速率、凋亡水平、基因表达标志物、细胞死亡率以及遗传标志物变化等指标，结果显示该化合物具有可观的治疗潜力，有待进一步开展临床前验证研究。本研究采用统计学方法评估其在癌症治疗策略中的潜在应用价值。本研究的实验周期为2024年初至2025年5月中旬，共使用60只两栖动物作为实验对象，其中30只接受两栖动物来源化合物处理，剩余30只给予生理盐水作为对照。实验数据采用SPSS 26.0软件进行分析，运用t检验与卡方检验进行统计学比较，以P<0.05作为差异具有统计学意义的判定标准。在为期12周的观察周期内，定期测量并记录肿瘤体积与瘤重；同时检测细胞增殖、凋亡水平以及部分遗传因子的活性变化。与对照组相比，给药组的肿瘤体积、瘤重及细胞分裂速率均显著降低（P=0.0005）。给药组动物的细胞死亡率显著升高（P=0.0001），且遗传标志物表达发生显著改变（P=0.0001）。该化合物作为潜在治疗制剂，为癌症治疗新策略的开发提供了一定支撑，但相关结果仍需进一步验证以确认其实际应用价值。本研究证实，两栖动物来源的化合物可缩小肿瘤体积、调控细胞增殖、促进细胞凋亡并影响基因表达，上述结果均表明该物质具有抗肿瘤活性。