Expression data from Myotonic dystrophy type 1 (DM1) patient-derived induced pluripotent cell. Expression data from Myotonic dystrophy type 1 (DM1) patient-derived induced pluripotent cell

DM1-iPSCs were generated from the peripheral blood of male adult patients. All clones were generated using episomal vectors. For skeletal muscle differentiation of the DM1-iPSCs, tet-on MYOD1 expression vector was transfected into DM1-iPSCs. To examine whether DM1-iPSC clones with different repeat sizes have acquired different characteristics, we isolated DM1-iPSC clones with different repeat sizes. We isolated 2 clones (S45 and S118) showing repeat size of 400~900, 2 clones (S14 and S24) showing repeat size of 1200~1600, and 2 clones (S1 and S16) showing repeat size of 1800~2800. Overall design: Cells were harvested at undifferentiated stages for RNA extraction and hybridization on Affymetrix microarrays. We performed gene expression profiles of 6 DM1-iPSC subclones and 1 healthy control iPS cell (normal).

DM1型诱导多能干细胞（DM1-iPSCs）由成年男性患者外周血诱导生成，所有克隆株均采用游离型载体（episomal vectors）构建获得。为诱导DM1-iPSCs向骨骼肌细胞分化，我们将携带tet-on调控系统的MYOD1表达载体转染至DM1-iPSCs中。为探究不同重复序列拷贝数的DM1-iPSC克隆是否存在差异化表型，我们分离获得了携带不同重复序列拷贝数的DM1-iPSC克隆株：其中2株重复序列拷贝数介于400~900（S45、S118）、2株介于1200~1600（S14、S24）以及2株介于1800~2800（S1、S16）。实验整体设计：于未分化阶段收集细胞，提取总RNA并进行Affymetrix基因芯片杂交检测。本研究对6株DM1-iPSC亚克隆及1株健康对照诱导多能干细胞（iPS cell，正常对照组）开展了基因表达谱分析。