Gene expression profiles in human bronchial epithelial cells exposure to cellulose nanofibrils and microcrystalline cellulose. Gene expression profiles in human bronchial epithelial cells exposure to cellulose nanofibrils and microcrystalline cellulose

To evaluate the biological impact of cellulose nanofibrils (CNFs) at the transcriptional level, we conducted whole-genome microarray analyses on human bronchial epithelial cells (BEAS-2B) exposed to CNFs with different physicochemical properties. The results were compared with those from exposures to microcrystalline cellulose (MCC). Overall design: Human bronchial epithelial cells (BEAS-2B) were exposed to eight types of cellulose nanofibrils (CNF1, CNF2, CNF3, CNF4, CNF5, CNF6, CNF7, and CNF8) at a concentration of 100 µg/mL, as well as MCC, for 48 hours. A vehicle control group (n=4) was also included. Cyanine-3-labeled cRNA generated from BEAS-2B cells exposed to CNFs and MCC was hybridized to a Whole Human Genome Microarray. Scanned images of the hybridized microarray slides were then analyzed numerically to assess the transcriptional-level biological effects of CNFs on BEAS-2B, in comparison with MCC exposures.

为评估纤维素纳米纤丝（cellulose nanofibrils，CNFs）在转录水平的生物学影响，本研究对暴露于不同物理化学性质CNFs的人支气管上皮细胞（BEAS-2B）开展全基因组微阵列分析，并将分析结果与暴露于微晶纤维素（microcrystalline cellulose，MCC）的样本进行对比。
实验整体设计：将人支气管上皮细胞（BEAS-2B）以100 μg/mL的浓度分别暴露于8种纤维素纳米纤丝（CNF1、CNF2、CNF3、CNF4、CNF5、CNF6、CNF7及CNF8）与微晶纤维素（MCC），暴露时长为48小时；同时设置溶剂对照组（n=4）。从暴露于CNFs与MCC的BEAS-2B细胞中制备的花青-3（Cyanine-3）标记互补RNA（complementary RNA，cRNA），与全人类基因组微阵列芯片进行杂交反应。随后对杂交后的微阵列芯片扫描图像进行数值化分析，以对比评估CNFs与MCC暴露对BEAS-2B细胞产生的转录水平生物学效应。