Positive regulation of Hedgehog signaling via phosphorylation of GLI2/GLI3 by DYRK2 kinase. Positive regulation of Hedgehog signaling via phosphorylation of GLI2/GLI3 by DYRK2 kinase

To understand the molecular mechanisms underlying developmental abnormalities in Dyrk2-/- mice, we performed comprehensive whole-genome RNA sequencing of the MEFs from wild-type and Dyrk2-/- embryos Overall design: We performed gene expression profiling analysis of RNA-seq data for wild-type and Dyrk2-/- MEFs in the presence of 100 nM SAG for 24 h as well as MEFs treated with siDyrk2 or siNegative (siNeg) in the presence of 100 nM SAG for 24 h

为阐明Dyrk2基因敲除（Dyrk2-/-）小鼠发育异常的潜在分子机制，我们对野生型与Dyrk2-/-胚胎来源的小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts, MEFs）开展了全基因组RNA测序分析。实验整体设计：我们针对经100 nM SAG处理24小时的野生型及Dyrk2-/- MEFs，以及经靶向Dyrk2的小干扰RNA（siDyrk2）或阴性对照小干扰RNA（siNegative, siNeg）处理并在100 nM SAG存在条件下培养24小时的MEFs的RNA测序数据，进行了基因表达谱分析。