CIL:45796, Homo sapiens. In Cell Image Library

Plate 24641 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

第24641号实验板——表型谱分析（Phenotypic profiling）旨在通过对每个样本的细胞表型开展多参数、基于特征的分析进行总结，使谱图间的相似性能够反映样本间的相似性。本图像集为测试基于图像的谱分析方法区分小分子化合物作用效果的能力提供了研究基础。

图像
本数据集的图像来源于经1600种已知生物活性化合物分别处理的U2OS细胞，采用六种标记物对七种细胞器进行染色（即细胞画谱检测法（cell-painting assay））。
本预实验共包含20块实验板，每块实验板设有384个孔位，每个孔位包含9个视场，总视场数达69120个。每个视场采用5个成像通道（即检测波长）进行成像，每个通道的图像均以独立的灰度图像文件存储，因此本数据集共包含345600个16位TIFF格式的图像文件。