Genome-wide analysis of gene expression in osteoclast precursor cells cultured for 3 days with either M-CSF or M-CSF + RANKL. Genome-wide analysis of gene expression in osteoclast precursor cells cultured for 3 days with either M-CSF or M-CSF + RANKL

Genome wide expression analysis of murine bone marrow osteoclast precursor cells that were cultured for 3 days either with macrophage colony stimulating factor (M-CSF) alone to remain as monocytes or M-CSF + receptor activator of NF-kB (RANKL) to differentiate down the osteoclast lineage. Results provide important information on genes that are regulated by RANKL in order to drive commitment to the osteoclast lineage. Overall design: RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Raw data are unavailable for this series. The UConn Health core facility where these arrays were processed no longer exists.

本数据集针对小鼠骨髓破骨细胞前体细胞开展全基因组表达分析：将细胞分别以仅添加巨噬细胞集落刺激因子（macrophage colony stimulating factor, M-CSF）的培养基培养3天以维持单核细胞状态，或以添加M-CSF联合核因子κB受体活化因子（receptor activator of NF-kB, RANKL）的培养基培养3天，使其向破骨细胞谱系定向分化。本研究结果可为解析RANKL调控破骨细胞谱系定向分化的相关基因提供重要参考依据。
实验设计：采用Trizol试剂提取核糖核酸（RNA），随后按照试剂盒配套操作规程，使用QIAGEN RNeasy迷你试剂盒完成RNA纯化与脱氧核糖核酸酶I（DNase I）处理；通过安捷伦生物分析仪（Agilent Bioanalyser）进行RNA质量质控。本数据集的原始数据未公开，原因是本次芯片处理所用的康涅狄格大学健康中心（UConn Health）核心实验室已不复存在。