The role of Prokineticin 2 in endometrial decidualization. The role of Prokineticin 2 in endometrial decidualization

Recurrent implantation failure(RIF) is an urgent problem to be solved in reproduction.Currently, endometrium decidualization damage is considered as one of the important causes, but the mechanism is still unclear. Prokineticin 2(PK2) is a newly discovered secreted protein with multiple biological functions, and its role in the endometrium is unknown.Our previous study showed that PK2 was significantly increased in RIF patients, but endometrial decidualization markers PRL and IGFBP1 were lower compared with the control group. We performed transcriptome sequencing, we found there were significant differences between the PK2 overexpression group and the control group. Differential genes mainly involved in decidualization process including adhesion, immunity, chemotaxis, ect. Overall design: Gene expression profile of PK2 overexpression group through lentivirus infection compared with control group in decidualized cells

复发性植入失败（Recurrent implantation failure, RIF）是生殖领域亟待解决的关键问题。目前，子宫内膜蜕膜化损伤被认为是其重要致病诱因之一，但具体分子机制仍未明确。运动素2（Prokineticin 2, PK2）是一类新发现的多功能分泌蛋白，其在子宫内膜中的生物学功能尚未阐明。本团队前期研究发现，RIF患者体内PK2的表达水平显著升高，而相较于对照组，患者子宫内膜蜕膜化标志物PRL与IGFBP1的表达水平却显著降低。本研究通过转录组测序分析，发现PK2过表达组与对照组的基因表达存在显著差异，这些差异基因主要参与蜕膜化相关的生物学过程，包括黏附、免疫、趋化等。整体实验设计：以蜕膜化细胞为研究对象，对比经慢病毒感染构建的PK2过表达组与对照组的基因表达谱。