Understanding adult human progenitor cells

Overarching aim is to achieve a greater understanding of the control of progenitor cells within the adult human retina within the normal and diseased retinal microenvironment. Specifically we will assess via our experimental designs: (i) the control of CD133+ retinal cell populations that display mitotic potential and differentiation and Keywords: comparative genomic hybridization using adult human CD133+ & CD133- retinal cells Adult human retina was processed to produce a cell suspension, CD133+ cells were magnetically isolated to produce cell populations of CD133+ and CD133- cells. 3 retinal samples were analysed for each cell population.

本研究的核心目标是深入解析正常及病变视网膜微环境中，成人视网膜内祖细胞的调控机制。具体而言，本研究将通过实验设计开展相关评估：(i) 对具备有丝分裂潜能与分化能力的CD133阳性（CD133+）视网膜细胞群体的调控模式。
关键词：采用成人CD133阳性与CD133阴性视网膜细胞的比较基因组杂交（comparative genomic hybridization）
本研究通过处理成人视网膜组织制备细胞悬液，采用磁分离法富集CD133阳性细胞，进而获得CD133阳性与CD133阴性两类细胞群体。每类细胞群体均使用3例视网膜样本完成分析。