Genome-wide identification of transcriptional targets for H4K12 lactylation in ccRCC cells

To investigate the genome-wide transcription targets of H4K12 lactylation in ccRCC cells, cleavage under targets and tagmentation (CUT&Tag) was performed in 786-O and Caki-1 cells using antibody against H4K12 lactylation. Following CUT&Tag, DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE. Overall design: CUT&Tag of H4K12 lactylation in 786-O and Caki-1 cells

为探究肾透明细胞癌（ccRCC）细胞中组蛋白H4赖氨酸12乳酸化（H4K12 lactylation）的全基因组转录靶点，本研究使用抗H4K12乳酸化抗体，在786-O与Caki-1细胞中开展靶向切割与标签化转座酶富集（CUT&Tag）实验。CUT&Tag实验完成后，采用无偏扩增条件对所得DNA进行扩增、标记，并通过Illumina NovaSeq 150PE平台完成测序。总体实验设计：在786-O与Caki-1细胞中开展H4K12乳酸化的CUT&Tag检测。