Holobiont response to thermal stress of two Pocillopora populations from contrasted thermal regimes

Two population of Pocillopora sp. coming from two constrated thermal environments (stable or variable) are compared for their bacterial compositions (16S/V3-V4 metabarcoding), Symbiodinium compositions (ITS2-metabarcoding) and gene expression (RNAseq by Illumina) in a Control (the mean temperature of warmer months in their natural environment for each population) and a stress condition (the maximal temperature before first sign of physiological collapses).本研究选取两个分别来自热环境对比鲜明的两类生境（稳定型与波动型）的杯形珊瑚属（Pocillopora sp.）种群，比较二者在对照组与胁迫组两种培养条件下的三项组学指标：细菌群落组成（采用16S/V3-V4元条形码测序（metabarcoding）技术）、虫黄藻（Symbiodinium）群落组成（采用ITS2元条形码测序技术）以及基因表达水平（采用Illumina平台RNA测序技术）。其中对照组温度设置为对应种群自然生境中暖季的平均温度，胁迫组温度则设置为对应种群首次出现生理衰退迹象前的最高温度。