mRNA analysis on peripheral blood mononuclear cells (PBMCs) performed by RNA-seq in patients with Bladder Cancer

Bladder cancer (BC) is the most common malignant tumor of the urinary tract, ranking 4th among men and 18th among women, and it is mainly divided into non-muscle invasive and muscle-invasive. Recent studies showed that changes in the transcriptome activity may contribute to carcinogenesis. Thus, the study of the transcriptome may allow the identification of additional differentially expressed cancer-related candidate genes and, consequently, a better understanding of the molecular basis of gene regulation in BC. This case-control study consists of a mRNA analysis performed by RNA-seq in 11 BC patients (11 males; mean age 63.00±9.88 years) and 19 age- and sex-matched healthy controls (19 males ; mean age 58.81±23.41 years) from publish dataset.

膀胱癌（Bladder cancer, BC）是泌尿系统最常见的恶性肿瘤，在男性恶性肿瘤中位列第4位，女性中位列第18位，主要分为非肌层浸润性膀胱癌与肌层浸润性膀胱癌两类。现有研究表明，转录组（transcriptome）活性的改变可能参与致癌进程，因此对转录组的研究可助力识别更多与癌症相关的差异表达候选基因，进而更深入地阐明膀胱癌基因调控的分子基础。本病例对照研究基于已发表的数据集，对11名膀胱癌患者（均为男性，平均年龄63.00±9.88岁）及19名年龄、性别匹配的健康对照个体（均为男性，平均年龄58.81±23.41岁）开展了RNA测序（RNA-seq）的mRNA分析。