Gene expression analysis of MCF12A human breast cultures exposed to ethanol or acetaldehyde [miRNA]. Homo sapiens

MCF12A monolayer cultures were exposed to ethanol or acetaldehyde for eiher 1 week or 4 weeks Total RNA samples were assayed for gene expression and for microRNA expression. MicroRNA expression was accomplished using 2 experiments. The first experiment included untreated control, ethanol treated (4 weeks at 2.5 mM) and acetaldehyde treated (4 weeks at 1 mM). The data are presented in order of control1 for the ethanol and acetaldehyde treated samples followed by the ethanol and acetaldehyde treated samples, and then the control2 for the soft agar selected sample followed by the soft agar selected sample. The microRNA names are included in separate columns. Overall design: Cells were cultured in DMEM/F12 in the presence of serum and growth factors. RNA was extracted using the miRVana kit and subjected to analysis of microRNAs.

将MCF12A单层培养物暴露于乙醇或乙醛中，处理时长为1周或4周。随后对总RNA样本开展基因表达与微小RNA（microRNA）表达水平检测。微小RNA表达检测通过两项实验完成：第一项实验包含未处理对照组、乙醇处理组（2.5 mM，处理4周）以及乙醛处理组（1 mM，处理4周）。数据排布顺序为：乙醇与乙醛处理样本的对照1、对应处理样本，随后为软琼脂筛选样本的对照2以及对应软琼脂筛选样本。微小RNA名称单独列于独立列中。整体实验设计：细胞于添加血清与生长因子的DMEM/F12培养基中培养；总RNA采用miRVana试剂盒提取，随后进行微小RNA表达分析。