P14 T cells from C57BL6J draining lymph node after vaccinia-GP33

CD90.1 T cells were isolated from draining lymph nodes of vaccinia-GP33 infected ears 45 days post infection P14 T cells from draining lymph nodes were MACS enriched for CD90.1, then flow sorted on CD90.1+, CD8+, CD44+, Live (DAPI-), CD45 IV- (extravascular). Enriched cells were then submitted to NYU genomics center for single cell RNA sequencing

在感染牛痘病毒-GP33（vaccinia-GP33）后的第45天，从感染小鼠的耳部引流淋巴结中分离P14 CD90.1阳性T细胞；随后将该引流淋巴结来源的P14 T细胞经磁激活细胞分选（Magnetic Activated Cell Sorting，MACS）富集CD90.1阳性细胞，再通过流式细胞术分选出符合CD90.1+、CD8+、CD44+表型的活细胞（DAPI染色阴性），且为CD45 IV-（血管外）的目标细胞亚群。富集后的细胞被送至纽约大学（NYU）基因组中心开展单细胞RNA测序（single cell RNA sequencing）。