Assessment of cow and farm level risk factors associated with Ureaplasma diversum in pasture-based dairy systems - A field study

ABSTRACT Potential risk factors for Ureaplasma diversum in the vaginal mucus of 1,238 dairy cows were included in a multivariate logistic regression model, based on the cow level (i.e., granular vulvovaginitis [+GVV], yearly milk production [4500 kg or more], pregnancy, predominance of Bos taurus [+Bos Taurus], score of corporal condition [at least 2.5], concomitant positivity for Escherichia coli [+E.coli]), and farm level i.e., milking room hygiene (-Milking room), dunghill location, and replacement female). Ureaplasma diversum was present in 41.1% of the samples. Independent risk factors for U. diversum were +GVV (odds ratio [OR], 1.31); +Mycoplasma spp (OR, 5.67); yearly milk production (4500 kg or more) (OR, 1.99); +Bos taurus (OR, 1.68); +E. coli (OR, 4.96); -milking room (OR, 2.31); and replacement females (OR, 1.89). Ureaplasma diversum vaginal colonization was strongly associated with Mycoplasma spp., E. coli, and number of pregnant cows.

摘要 本研究将1238头奶牛阴道黏液中检测多样性脲原体（Ureaplasma diversum）的潜在危险因素，纳入以个体牛为单元的多因素logistic回归模型（multivariate logistic regression model），分析维度涵盖个体牛与牧场两个层面：个体牛层面因素包括颗粒性外阴阴道炎（granular vulvovaginitis, GVV）阳性、年泌乳量≥4500 kg、妊娠状态、普通牛（Bos taurus）占优、体况评分≥2.5，以及大肠杆菌（Escherichia coli, E. coli）阳性；牧场层面因素包括挤奶厅卫生不达标（-Milking room）、粪堆选址，以及后备母牛饲养情况。本研究纳入的样本中，41.1%检出多样性脲原体。多样性脲原体感染的独立危险因素分别为：+GVV（比值比（odds ratio, OR）=1.31）、支原体属（Mycoplasma spp.）阳性（OR=5.67）、年泌乳量≥4500 kg（OR=1.99）、普通牛占优（OR=1.68）、大肠杆菌阳性（OR=4.96）、挤奶厅卫生不达标（OR=2.31）以及饲养后备母牛（OR=1.89）。奶牛阴道多样性脲原体定植与支原体属阳性、大肠杆菌阳性以及妊娠母牛数量存在显著关联。