Characterization of carboplatin loaded micro-nanostructured porous granular material in a biphasic calcium phosphate ceramic system

ABSTRACT The hydroxyapatite/beta-tricalcium phosphate (HA/β-TCP) bioceramics, has been reported to be attractive for biomedical applications, used as bone tissue substitute materials. These biomaterials exhibited excellent biocompatibility and interconnected microporous microstructure that provides suitable environments for a controlled drug delivery system in bone treatments. The characterization study of the biphasic granular material 60% HA/40% β-TCP, was performed before and after loading the carboplatin using a high vacuum method. The drug was loaded at concentrations of 1 mg/g, 5 mg/g, 10 mg/g and 20 mg/g in a biphasic granular biomaterial. The morphological, microstructural, crystallographic and chemical characterization of the granulated biomaterial was investigated. SEM analysis showed structures identified as drugs precipitates on the structure of the biomaterial. The drug has been identified using X-ray technique, also. FTIR analysis indicated OH- and PO43- from calcium phosphates and Pt-N and NH3 vibrational bands from carboplatin molecules. The results showed that the high vacuum method was efficient for the incorporation of carboplatin in micropore structure of the granular biomaterial.

摘要 羟基磷灰石/β-磷酸三钙（hydroxyapatite/beta-tricalcium phosphate, HA/β-TCP）生物陶瓷作为骨组织替代材料，已有研究表明其在生物医学领域具备良好应用前景。该类生物材料具备优异的生物相容性与相互连通的微孔微观结构，可为骨治疗中的控释给药系统提供适宜的载体环境。本研究针对60%羟基磷灰石/40%β-磷酸三钙（60% HA/40% β-TCP）双相颗粒材料，在采用高真空法负载卡铂前后均开展了表征分析。实验中，卡铂在该双相颗粒生物材料上的负载浓度分别设置为1 mg/g、5 mg/g、10 mg/g与20 mg/g。本研究对该颗粒状生物材料的形貌、微观结构、晶体学与化学特性展开了表征分析。扫描电子显微镜（Scanning Electron Microscope, SEM）分析结果显示，生物材料表面出现了可识别为药物沉淀物的结构。此外，本研究还通过X射线技术完成了对该药物的定性鉴定。傅里叶变换红外光谱（Fourier Transform Infrared Spectroscopy, FTIR）分析结果表明，样品中存在磷酸钙类物质的OH⁻与PO₄³⁻特征振动峰，以及卡铂分子的Pt-N和NH₃振动峰。实验结果证实，高真空法可有效实现卡铂在颗粒状生物材料微孔结构中的负载。