affy_popsec_nancy_pophydro_roots_poplar -Molecular bases of acclimation and adaptation to water deficit in poplar. Populus x canadensis

affy_popsec_nancy_pophydro_roots_poplar - This project aims to identify genes of interest for control of root growth in response to water deficit in a tree species: poplar. We look for genes and gene expression networks related to drought stress. We intend to analyse the transcriptome in root apices of cuttings grown in hydroponics and osmotically stressed with PEG. Root apex is the location of root elongation and these analyses intend to identify genes involved in the control of cell expansion and thus of root elongation. Indeed, root growth maintenance in response to water shortage contributes to plant tolerance to water deficit.-Poplar cuttings (cv Soligo) were grown in hydroponics. A moderate water deficit was applied by adding PEG to the nutrient solution (200g/l). After 3 days, the apex (1 cm-long) of the roots of each cutting were collected. For control and stressed treatments, RNAs were extracted from two pools of 5 to 9 roots (issued from 2 to 5 cuttings). A pool is considered as one biological replicate and corresponds to one Affymetrix slide. Keywords: treated vs untreated comparison Overall design: 4 arrays - poplar

affy_popsec_nancy_pophydro_roots_poplar — 本项目旨在鉴定林木物种杨树中响应水分亏缺调控根系生长的目标基因，挖掘与干旱胁迫相关的基因及基因表达网络。我们将对水培培养、经聚乙二醇（PEG）渗透胁迫处理的插条根尖开展转录组（transcriptome）分析。根尖是根系伸长的核心区域，本分析旨在鉴定参与细胞扩张调控、进而调控根系伸长的相关基因。事实上，水分匮乏条件下根系生长的维持，可有效提升植物对水分亏缺的耐受能力。
实验采用杨树插条（品种Soligo），以水培方式培养。通过向营养液中添加200g/L的PEG施加中度水分胁迫。处理3天后，采集每株插条的1cm长根尖。对照组与胁迫组的RNA均提取自5~9条根组成的混合样本（取自2~5株插条），每个混合样本视为1个生物学重复，对应1张Affymetrix基因芯片。
关键词：处理组与对照组比较
整体实验设计：4张杨树基因芯片