Detection of a deletion strongly associated with the fruit flat shape in peach.

This study reports a 10Kb deletion affecting the gene PRUPE.6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety ‘UFO-4’, revealing that the mutation affected the flat associated allele (S).

本研究报道了一处影响基因PRUPE.6G281100的10千碱基对（10Kb）缺失，该基因位于UDP98-412上游400千碱基对（400 Kb）处，且与目标性状共分离。该基因属于富亮氨酸重复序列类受体激酶（leucine-rich repeat receptor-like kinase, LRR-RLK），与油菜素内酯不敏感1相关受体激酶1（Brassinosteroid insensitive 1-associated receptor kinase 1, BAK1）类群为直系同源基因。适用于分子标记辅助选择（Marker-Assisted Selection, MAS）的聚合酶链式反应（Polymerase Chain Reaction, PCR）标记，在246份栽培品种构成的群体中验证了其与该性状的强关联。研究人员利用这些标记对源自扁果品种‘UFO-4’体细胞突变获得的圆果材料的DNA进行检测，结果显示该突变影响了与扁果性状相关的等位基因（S）。