Hydration status of geriatric patients is associated with changes of serum proteome especially of proteins involved in coagulation

Due to multifactorial reasons, such as decreased thirst and decreased total body water, elderly patients are vulnerable to dehydration. The study aims to investigate whether moderate dehydration or hyperhydration affects the blood proteome. Blood samples, medication and bioelectrical impedance analysis (BIA) details were collected from 131 geriatric patients (77 women, 54 men). Based on an evaluation by Bioelectrical Impedance Vector Analyses (BIVA) of this cohort, for each hydration status (dehydrated, hyperhydrated and control), five appropriate blood serum samples for both male and female were analyzed by LC-MS. Overall, 262 proteins for female patients and 293 proteins for male patients could be quantified. 38 proteins had significantly different abundance, showing that hydration status does indeed affect the serum proteome. Protein enrichment analysis of the affected proteins revealed “Wound Healing” and “Keratinization” as the two main biological processes being dysregulated. Especially, proteins involved in clot formation are affected by hydration status.

由于口渴感减退、总体体液减少等多因素影响，老年患者更易发生脱水。本研究旨在探究中度脱水或体液过多是否会对血液蛋白质组产生影响。研究团队从131名老年患者（其中女性77名、男性54名）中采集了血液样本、用药情况及生物电阻抗分析（Bioelectrical Impedance Analysis, BIA）的相关细节。基于对该队列的生物电阻抗向量分析（Bioelectrical Impedance Vector Analyses, BIVA）评估结果，针对脱水、体液过多及正常对照三种水合状态，分别为男女受试者各选取5份合格血清样本，采用液相色谱-质谱联用（Liquid Chromatography-Mass Spectrometry, LC-MS）技术进行分析。最终，共实现对女性患者的262种蛋白质、男性患者的293种蛋白质的定量检测。其中38种蛋白质的丰度存在显著差异，表明水合状态确实会影响血清蛋白质组。对差异蛋白质进行富集分析后发现，伤口愈合与角化是两个主要出现异常调控的生物学过程。尤为值得注意的是，参与凝血过程的蛋白质会受到水合状态的影响。