Ablation of Histone Demethylase KDM5B in Melanoma Augments Anti-Tumor Immunity through Upregulation of Retroelements [ATAC-seq]

Purpose: Advanced melanoma patients have poor prognosis. Although immune checkpoint blockade has revolutionized treatment for melanoma patients, majority of patients do not respond. The goal of this research is to evaluate whether epigenetic therapy targeting KDM5B could overcome resistance to immunotherapy. Methods: ATAC-Seq analysis of mouse melanoma cell line YUMMER1.7. Results: We identified that KDM5B directly bind to retreoelements to repress their expression. Conclusions: Our work characterized ablation of histone demethylase KDM5B in melanoma augments anti-tumor immunity through Upregulation of retroelements. Overall design: ATAC-seq analysis in mouse melanoma cell lines

### 研究目的
晚期黑色素瘤患者预后较差。尽管免疫检查点阻断（immune checkpoint blockade）疗法已彻底革新了黑色素瘤的临床治疗方案，但多数患者仍无法对该疗法产生应答。本研究旨在评估靶向KDM5B的表观遗传治疗（epigenetic therapy）能否克服肿瘤对免疫治疗的耐药性。
### 研究方法
对小鼠黑色素瘤细胞系YUMMER1.7开展ATAC测序（ATAC-Seq）分析。
### 研究结果
本研究发现KDM5B可直接结合逆转录元件（retroelements）并抑制其表达。
### 研究结论
本研究明确，黑色素瘤中组蛋白去甲基化酶（histone demethylase）KDM5B的功能缺失可通过上调逆转录元件的表达，增强抗肿瘤免疫应答。
### 整体实验设计
针对小鼠黑色素瘤细胞系的ATAC测序分析。