lncRNA expression reveals the potential regulatory roles in ox-LDL induced macrophage-derived foam cells

The goals of this study are to analysis the potential regulatory roles in ox-LDL induced macrophage-derived foam cells by lncRNA. A total of 50688 mRNA and 1514 lncRNA were found, and including 51 lncRNAs and 1730 mRNAs were significantly dysregulated in the model group (p-adjust < 0.05 and |log2FC| > 2). Then the result of GO and KEGG pathways showed that this dysregulated genes were significantly enriched in molecular function and biological processes and they were associated cell proliferation, cell apoptosis and inflammatory response. Additionally, the results of the lncRNA-mRNA co-expression network showed that 15 mRNAs and 9 lncRNAs were identified as the key regulatory factors, which may play crucial roles in AS development. Especially, the lncRNAs, Brip1os, Gm15472, Gm16586, Gm20703, and AU020206 could be seen as the potential biomarkers in macrophage-derived foam cells. This study revealed the dysregulated lncRNAs and mRNAs in AS development and the lncRNAs, Brip1os, Gm15472, Gm16586, Gm20703, and AU020206 were identified as the key regulatory factors. The dysregulated genes may be consider as the potential diagnostic biomarkers of AS, and it needs more verification in future studies. lncRNA and mRNA profiles of ox-LDL induced macrophage cells

本研究旨在探讨长链非编码RNA（long non-coding RNA, lncRNA）在氧化低密度脂蛋白（oxidized low-density lipoprotein, ox-LDL）诱导的巨噬细胞源性泡沫细胞中的潜在调控作用。共检测到50688条信使RNA（messenger RNA, mRNA）及1514条lncRNA，其中模型组内有51条lncRNA与1730条mRNA存在显著异常表达（校正后P值<0.05且|log2FC|>2）。随后的基因本体（Gene Ontology, GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路分析结果显示，上述差异表达基因显著富集于分子功能与生物学过程范畴，且与细胞增殖、细胞凋亡及炎症反应密切相关。此外，lncRNA-mRNA共表达网络分析结果表明，15条mRNA与9条lncRNA被鉴定为关键调控因子，可能在动脉粥样硬化（atherosclerosis, AS）的发生发展中发挥关键作用。尤为值得注意的是，Brip1os、Gm15472、Gm16586、Gm20703及AU020206等lncRNA可作为巨噬细胞源性泡沫细胞的潜在生物标志物。本研究揭示了动脉粥样硬化发生发展过程中异常表达的lncRNA与mRNA，上述Brip1os、Gm15472、Gm16586、Gm20703及AU020206等lncRNA被鉴定为关键调控因子。这些差异表达基因可作为动脉粥样硬化的潜在诊断生物标志物，未来仍需开展更多验证研究。本数据集为ox-LDL诱导的巨噬细胞的lncRNA与mRNA表达谱。