Xylella fastidiosa subsp. multiplex strain:CFBP8417 Genome sequencing and assembly

Xylella fastidiosa, a bacterial plant pathogen confined in the past to the Americas, recently emerged in Europe. It was first reported in natural settings in France from Polygala myrtifolia shrubs in Corsica in July 2015 and in south-eastern mainland France in October 2015. These ornamentals were symptomatic and presented severe leaf scorches. All contaminated samples from France presented bacteria belonging to X. fastidiosa subsp. multiplex, based on multiprimer PCR (Hernandez-Martinez et al., Phytopathol. 2006) analyses run on DNAs extracted from the infected plant samples. A MLST scheme was used to type these bacteria which are characterized by two X. fastidiosa subsp. multiplex bona fide sequence types, ST6 and ST7. Three strains were isolated from Polygala myrtifolia (CFBP 8416; ST7) and Spartium junceum (CFBP 8417 and CFBP 8418; ST6). The genomes of these strains were sequenced using the Illumina Mi-Seq technology.

苛养木杆菌（Xylella fastidiosa）是一种曾仅局限于美洲的植物病原细菌，近年在欧洲出现并扩散。该菌于2015年7月首次在法国科西嘉岛的桃金娘叶远志（Polygala myrtifolia）灌丛自然生境中被报道，同年10月又在法国本土东南部地区被发现。上述观赏植物表现出明显病害症状，呈现严重的叶焦枯病征。通过对感病植物样本提取的DNA进行多引物PCR（multiprimer PCR）分析（Hernandez-Martinez等，《植物病理学》，2006年），法国所有带菌样本中的病原菌均归属于苛养木杆菌多寄主亚种（X. fastidiosa subsp. multiplex）。研究采用多位点序列分型（MLST, Multi-Locus Sequence Typing）方案对这些病原菌进行分型，结果显示其属于两个正宗的苛养木杆菌多寄主亚种序列型：ST6与ST7。研究人员从桃金娘叶远志（CFBP 8416；ST7）和鹰爪豆（Spartium junceum）中分离得到3株菌株：其中CFBP 8416分离自桃金娘叶远志（ST7型），CFBP 8417与CFBP 8418分离自鹰爪豆（均为ST6型）。上述菌株的基因组均采用Illumina MiSeq测序技术完成测序。