Hepatic ARID3A facilitates liver cancer malignancy by cooperating with CEP131 to regulate an embryonic stem cell-like gene signature

Huh 7 cells were infected with lentivirus or transfected with siRNA using Lipofectamine RNAiMAX to overexpress or knockdown indicated genes. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed using indicated antibodies to study the H3K9me2 status after certain treatment in Huh7 cells. Cleavage Under Targets and Tagmentation (CUT&Tag) was performed to explore the chromatin binding sites of ARID3A. Whole-transcriptome sequencing (RNA-Seq) was performed to identify the downstream target genes regulated by ARID3A or KDM3A in Huh 7 cells. Huh 7 cells were infected with lentivirus or transfected with siRNA using Lipofectamine RNAiMAX to overexpress or knockdown indicated genes. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed using indicated antibodies to study the H3K9me2 status after certain treatment in Huh7 cells. Cleavage Under Targets and Tagmentation (CUT&Tag) was performed to explore the chromatin binding sites of ARID3A. Whole-transcriptome sequencing (RNA-Seq) was performed to identify the downstream target genes regulated by ARID3A or KDM3A in Huh 7 cells.

以慢病毒（lentivirus）感染Huh7细胞，或采用Lipofectamine RNAiMAX转染小干扰RNA（siRNA），以过表达或敲低指定基因。使用指定抗体开展染色质免疫共沉淀测序（ChIP-seq），以探究Huh7细胞经特定处理后的H3K9me2修饰状态。开展靶标切割与标签化测序（CUT&Tag）实验，以解析ARID3A的染色质结合位点。开展全转录组测序（RNA-Seq），以鉴定Huh7细胞中受ARID3A或KDM3A调控的下游靶基因。以慢病毒（lentivirus）感染Huh7细胞，或采用Lipofectamine RNAiMAX转染小干扰RNA（siRNA），以过表达或敲低指定基因。使用指定抗体开展染色质免疫共沉淀测序（ChIP-seq），以探究Huh7细胞经特定处理后的H3K9me2修饰状态。开展靶标切割与标签化测序（CUT&Tag）实验，以解析ARID3A的染色质结合位点。开展全转录组测序（RNA-Seq），以鉴定Huh7细胞中受ARID3A或KDM3A调控的下游靶基因。