POF (MO 459).2-4 hr OR embryo

modENCODE_submission_3952 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 2-4hr OR; Genotype: wild type; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody POF (MO 459) (target is POF); Developmental Stage Embryo 2-4hr OR

modENCODE_submission_3952 本提交源自Gary Karpen主持的modENCODE项目。如需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究旨在确定黑腹果蝇（Drosophila melanogaster）基因组内主要组蛋白修饰的分布位点。所研究的修饰参与了DNA复制、基因表达、基因沉默及遗传等核心染色体生物学功能。我们将采用基因组平铺阵列开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。初始阶段将使用三种细胞系与两个胚胎阶段的染色质进行位点定位分析，后续将把部分靶蛋白的分析范围扩展至另外四种动物组织/发育阶段。关于本数据的使用条款与条件，请参阅http://www.genome.gov/27528022与http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

实验类型：ChIP-chip。

生物来源：菌株：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；发育阶段：胚胎2-4小时 Oregon-R；基因型：野生型；
生物学重复次数：4；
实验因素：Oregon-R菌株（官方名称：Oregon-R-modENCODE，基因型：野生型）；抗体POF（MO 459）（靶标为POF）；发育阶段：胚胎2-4小时 Oregon-R