RNA-seq of bone marrow neutrophil treated PGE2 or Vehicle

Purpose: Investigate the neutrophils could exspress different mRNA de novo from PGE2 stimulation. Methods: Isolated bone marrow neutrophils with Percoll gradient are treated with 10 µM of PGE2 for 24 hours. Results: Qualified sequence reads per sample to the mouse genome (mm10) with Bowtie2 or STAR were processed with StringTie and identified genes. Conclusion : 1,359 genes were significantly different in PGE2 treated bone marrow neutrophils from vehicle treated ones. Overall design: RNA-seq analysis of neutrophils isolated from bone marrow treated 10µM PGE2 or DMSO vehicle for 24 hours. 3 biological replicates.

研究目的：探究中性粒细胞经前列腺素E2（PGE2）刺激后，能否从头合成差异表达的信使RNA（mRNA）。
实验方法：采用Percoll密度梯度离心法分离骨髓中性粒细胞，以10 μM PGE2处理24小时。
结果：对每个样本中经Bowtie2或STAR比对至小鼠基因组（mm10）的合格序列读段，采用StringTie进行转录组装与基因鉴定。
研究结论：经PGE2处理的骨髓中性粒细胞中，共有1359个基因与溶剂对照处理组存在显著表达差异。
实验整体设计：对经10 μM PGE2或二甲基亚砜（DMSO）溶剂对照处理24小时的骨髓分离中性粒细胞开展RNA测序（RNA-seq）分析，共设置3次生物学重复。