有/无葡萄糖培养的MDSC转录组芯片

纯化的CD34+细胞通过造血干细胞扩增培养基培养7-8天后，在有或者无葡萄糖的条件下进行G-CSF、GM-CSF刺激后，使用TRIZOL法提取总RNA。通过Agilent Bioanalyzer 2100检测RNA质量后，进一步用RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany)进行纯化。在合作公司通过Agilent-014850 Whole Human Genome Microarray 4x44K G4112F转录组芯片分析样本的转录组数据，并进行数据处理和差异基因分析，得到的数据样本通过实时荧光定量聚合酶链锁反应（RT-PCR）验证。

Purified CD34+ cells were cultured in hematopoietic stem cell expansion medium for 7–8 days, then stimulated with G-CSF and GM-CSF under glucose-supplemented or glucose-free conditions. Total RNA was extracted using the TRIZOL method, followed by RNA quality assessment with the Agilent Bioanalyzer 2100. The RNA was further purified using the RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany). Transcriptomic profiling of the samples was conducted at the collaborating company using the Agilent-014850 Whole Human Genome Microarray 4x44K G4112F, with subsequent data processing and differential gene expression analysis performed. The resulting transcriptomic dataset was validated via quantitative real-time polymerase chain reaction (RT-PCR).