datasheet3_Transcriptomic and Metabolomic Profiling Reveals the Protective Effect of Acanthopanax senticosus (Rupr. & Maxim.) Harms Combined With Gastrodia elata Blume on Cerebral Ischemia-Reperfusion Injury.xlsx

The effects of current treatment strategies used in ischemic stroke are weakened by cerebral ischemia-reperfusion (CIR) injury. Suitable treatment regimens targeting CIR injury are still lacking. Two herbs, namely, Acanthopanax senticosus (Rupr. & Maxim.) Harms (ASE) and Gastrodia elata Blume (GEB), have been used as traditional Chinese medicine and are indicated in the treatment of stroke and cerebrovascular diseases. However, there are no studies that report the effects of ASE combined with GEB in the treatment of CIR injury. In this study, we used the Zea Longa method to induce CIR injury in male Wistar rats. Results of the pharmacodynamic studies revealed that co-administration of ASE and GEB may improve neuronal injury and prevent neuronal apoptosis by reducing oxidative stress and inflammation, and also help prevent CIR injury. On the basis of our hypothesis, we combined the results from transcriptomic and metabonomic analyses and found that ASE and GEB could prevent CIR injury by targeting phenylalanine, pyrimidine, methionine, and sphingolipid metabolism. Therefore, our study provides the basis for the compatibility and efficacy of ASE and GEB.

当前缺血性脑卒中的临床治疗策略，其疗效会因脑缺血再灌注（cerebral ischemia-reperfusion, CIR）损伤而受到削弱。目前仍缺乏针对CIR损伤的有效治疗方案。两种中药材——刺五加（Acanthopanax senticosus (Rupr. & Maxim.) Harms, ASE）与天麻（Gastrodia elata Blume, GEB）——作为传统中药被广泛应用于临床，可用于脑卒中及脑血管疾病的治疗。然而，目前尚无研究报道刺五加与天麻联用对CIR损伤的治疗作用。本研究采用Zea Longa线栓法构建雄性Wistar大鼠脑缺血再灌注损伤模型。药效学研究结果显示，刺五加与天麻联用可通过减轻氧化应激与炎症反应，改善神经元损伤、抑制神经元凋亡，从而对脑缺血再灌注损伤起到保护作用。基于本研究的预设假说，我们整合转录组学与代谢组学分析结果，发现刺五加与天麻可通过靶向调控苯丙氨酸代谢、嘧啶代谢、甲硫氨酸代谢及鞘脂代谢通路，发挥脑缺血再灌注损伤的保护作用。综上，本研究为刺五加与天麻的配伍应用及其药效机制提供了实验基础。