MINC Regulates Pervasive Transcription in Yeast and Mammals

Purpose: We want to know whether MINC(Mot1-Ino80C-NC2) suppress pervasive transcription at both euchromatin and heterochromatin Using next generation sequencing we show that Mot1, Ino80 chromatin remodeling complex (Ino80C), and NC2 (MINC) colocalize on chromatin and cooperate to suppress pervasive transcription in S. cerevisiae and murine embryonic stem cells (mESCs). Conclusion: Our ChIP-Seq and mRNA-Seq data show that MINC regulates pervasive transcription in yeast and mammals Here we use ChIP-seq to identify the genome-wide location of Mot1 , Ino80 and Nc2 both in yeast and mouse. Also we use anchor away and knock out in yeast and knock down in mouse to investigate the function of MINC in regulation of pervasive transcription .

研究目的：本研究旨在探究MINC（Mot1-Ino80C-NC2）是否可在常染色质（euchromatin）与异染色质（heterochromatin）中抑制广泛转录（pervasive transcription）。通过下一代测序技术，我们证实：Mot1、Ino80染色质重塑复合物（Ino80 chromatin remodeling complex, Ino80C）与NC2共同组成MINC，三者在染色质上共定位，并协同抑制酿酒酵母（S. cerevisiae）及小鼠胚胎干细胞（mESCs）中的广泛转录。
研究结论：本研究的染色质免疫共沉淀测序（ChIP-Seq）与信使RNA测序（mRNA-Seq）数据表明，MINC可调控酵母与哺乳动物中的广泛转录。本研究通过染色质免疫共沉淀测序（ChIP-seq），鉴定了Mot1、Ino80与NC2在酵母及小鼠基因组中的全基因组定位情况；同时在酵母中采用锚定撤离技术（anchor away）与基因敲除（knock out）手段、在小鼠中采用基因敲低（knock down）手段，探究MINC在广泛转录调控中的功能。