Additional file 1 of Sexual-biased gene expression of olfactory-related genes in the antennae of Conogethes pinicolalis (Lepidoptera: Crambidae)

Additional file 1: Figure S1. A) functional annotation of assembled sequences based on gene ontology (GO) categorization; b) EuKaryotic of orthologous groups (KOG) classification; c) is KEGG pathway annotation of the transcriptome. Figure S2. Quantitative RT-qPCR expression levels of olfactory genes from female and male moth of C. pinicolalis. The expression levels were statistically significant (t-test, *P < 0.05, **P < 0.01, ***P < 0.001, NS: not significant). Figure S3. Phylogenetic relationship of olfactory-related gene from C. pinicolalis and other insects. A: ORs, B: IRs, C: CSPs. Red font represents the genes from C. pinicolalis; Cpun, Ofur, Bmor, Cmed, Ehip, Harm and Mcin are the abbreviation of C. punctiferalis, O. furnacalis, B. mori, Cnaphalocrocis medinalis, Eogystia hippophaecolus, Helicoverpa armigera, Macrocentrus cingulum, respectively.

附加文件1：补充图S1。A）基于基因本体（Gene Ontology, GO）分类的组装序列功能注释；B）真核生物同源群簇（EuKaryotic Orthologous Groups, KOG）分类；C）该转录组的京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路注释。补充图S2：松梢螟（C. pinicolalis）雌雄成虫嗅觉基因的实时定量反转录聚合酶链式反应（qRT-qPCR）表达水平。该表达差异具有统计学显著性（t检验，*P < 0.05，**P < 0.01，***P < 0.001，NS：无显著性差异）。补充图S3：松梢螟与其他昆虫嗅觉相关基因的系统发育关系。A：嗅觉受体（Odorant Receptors, ORs），B：离子型受体（Ionotropic Receptors, IRs），C：化学感受蛋白（Chemosensory Proteins, CSPs）。红色字体代表松梢螟的相关基因；Cpun、Ofur、Bmor、Cmed、Ehip、Harm及Mcin分别为桃蛀螟（Conogethes punctiferalis）、亚洲玉米螟（Ostrinia furnacalis）、家蚕（Bombyx mori）、稻纵卷叶螟（Cnaphalocrocis medinalis）、沙棘木蠹蛾（Eogystia hippophaecolus）、棉铃虫（Helicoverpa armigera）以及螟蛉悬茧蜂（Macrocentrus cingulum）的缩写。