Genes used for in vitro validation with corresponding targeting compounds and other uniquely essential genes.

We identified 7 uniquely essential genes to stomach associated pathogens (thyX, tktA, mqo, ndk, aroE, cdh, fumC) and reported their uniquely essential percentages. Two genes, fabF and fabZ are not uniquely essential genes, but are used as positive controls in the in vitro validation assay. Gene products, pathways that the gene is involved in are reported for all 9 genes, and the inhibitor, and inhibitor’s chemical formula are provided for the 3 genes that were used for in vitro validation and were not researched for genes not used for in vitro validation.

本研究针对胃部相关病原菌，筛选得到7个独有必需基因（thyX、tktA、mqo、ndk、aroE、cdh、fumC），并报告了各基因的独有必需性比例。另有fabF与fabZ两个基因并非独有必需基因，但作为阳性对照应用于体外验证实验中。针对全部9个基因，本研究均报告了其编码的基因产物及参与的代谢通路；其中仅针对3个用于体外验证的基因，提供了其所对应的抑制剂及该抑制剂的化学分子式，未针对非体外验证基因开展相关研究。