Pond water SIP fraction microbial communities from Walnut Creek, CA, United States - JGIPond_E_60pct_20ng_2_1.693 metagenome

SIP metagenome fraction of pond sample from Joint Genome Institute, Walnut Creek spiked with Pseudomonas putida & Escherichia coli bacterial isolates (for each isolate 20ng of DNA labeled at 60pct with C13 were added); replicate number 2. This fraction has a SIP fraction number 16 and DNA density of 1.693 g/uL. This is part of a larger study to identify the limit of detection of JGI SIP metagenomics pipeline.

本数据集为美国联合基因组研究所（Joint Genome Institute, JGI）核桃溪池塘样品的稳定同位素探针（Stable Isotope Probing, SIP）宏基因组组分，实验中掺入了恶臭假单胞菌（Pseudomonas putida）与大肠杆菌（Escherichia coli）细菌分离株：每株分离株均加入20ng经60%比例碳13（C13）标记的DNA，实验重复次数为2。该组分的SIP组分编号为16，DNA密度为1.693 g/uL。本数据集属于一项旨在明确JGI SIP宏基因组分析流程检出限的大型研究的组成部分。