Quantitative mass spectrometry analysis of intestinal organoids

Organoids have the potential to bridge 3D cell culture to tissue physiology by providing a model resembling in vivo organs. We propose here a SILAC method to measure protein expression changes in intestinal organoids under different experimental conditions. With the combined use of quantitative mass spectrometry, SILAC and organoid culture, we validated the approach and showed that large-scale proteome variations can be measured in an “organ-like” system under variable conditions.

类器官（Organoids）可通过构建模拟活体器官的模型，搭建三维细胞培养与组织生理学研究之间的桥梁。本文提出一种稳定同位素细胞氨基酸标记法（SILAC），用于定量检测不同实验条件下肠道类器官内的蛋白质表达变化。通过联合应用定量质谱法、SILAC与类器官培养技术，本研究对该方法进行了验证，并证实可在可变实验条件下的"organ-like"系统中检测到大规模蛋白质组（Proteome）变化。