Unparalleled mitochondrial heteroplasmy and Wolbachia co-infection in the non-model bee, Amphylaeus morosus

Datasets associated with Davies et al. 2022 "Unparalleled mitochondrial heteroplasmy and Wolbachia co-infection in the non-model bee, Amphylaeus morosus", Current Research in Insect Science.We describe widespread and conserved mitochondrial heteroplasmy in Australian native bee, Amphylaeus morosus and the co-occurring co-infection of the bacterial endosymbiont, Wolbachia across the species' distribution.Amphylaeus_iontorrent_mtgenome.zip = compressed folder of the partial mitogenome alignment and raw data for a single Amphylaeus morosus individual with DNA sequence data generated using Ion Torrent shotgun sequencing.Amphylaeus_sangerseq_mt-coi-gene.zip = compressed folder of DNA chromatograms of the (heteroplasmic) mitochondrial COI gene of 70 Amphylaeus morosus individuals with DNA sequences generated using Sanger sequencing.Wolbachia_BEAST_phylog_log.zip = compressed folder of DNA alignment and BEAST2 phylogenetic log and output files for the phylogenetic analysis of the Wolbachia COI-like and wsp genes from host, Amphylaeus morosus.Wolbachia_cloning.zip = compressed folder of DNA chromatograms of the cloning products of two Amphylaeus morosus individuals for the two Wolbachia gene regions wsp and COI-like, generated using Sanger sequencing.Wolbachia_sangerseq_coi-like-gene.zip = compressed folder of DNA chromatograms of the Wolbachia COI-like gene region (with heterogeneious base pairs indicating a bacterial co-infection) of 27 Amphylaeus morosus individuals, with DNA sequences generated using Sanger sequencing.

与Davies等人2022年发表于《昆虫科学当代研究》（Current Research in Insect Science）的论文《非模式蜂类Amphylaeus morosus中前所未有的线粒体异质性与沃尔巴克氏体（Wolbachia）共感染》相关的数据集。我们描述了澳大利亚本土蜂类Amphylaeus morosus中广泛且保守的线粒体异质性，以及该物种分布范围内普遍存在的细菌内共生体沃尔巴克氏体（Wolbachia）共感染现象。
Amphylaeus_iontorrent_mtgenome.zip = 压缩文件夹，包含单个Amphylaeus morosus个体的部分线粒体基因组比对结果和原始数据，其DNA序列数据通过Ion Torrent鸟枪法测序生成。
Amphylaeus_sangerseq_mt-coi-gene.zip = 压缩文件夹，包含70个Amphylaeus morosus个体的（异质性）线粒体COI基因的DNA色谱图，其DNA序列通过Sanger测序生成。
Wolbachia_BEAST_phylog_log.zip = 压缩文件夹，包含针对宿主Amphylaeus morosus体内沃尔巴克氏体的COI-like基因和wsp基因进行系统发育分析的DNA比对结果、BEAST2系统发育日志及输出文件。
Wolbachia_cloning.zip = 压缩文件夹，包含两个Amphylaeus morosus个体中沃尔巴克氏体的wsp和COI-like两个基因区域克隆产物的DNA色谱图，通过Sanger测序生成。
Wolbachia_sangerseq_coi-like-gene.zip = 压缩文件夹，包含27个Amphylaeus morosus个体中沃尔巴克氏体COI-like基因区域的DNA色谱图（其中异质性碱基对表明存在细菌共感染），其DNA序列通过Sanger测序生成。