microRNA expression data from peripheral blood mononuclear cell in patients with type 1 diabetes compared with normal controls.

Type 1 diabetes mellitus (T1D) is a common autoimmune disease mediated by autoimmune attack against pancreatic b cells. It has been reported that dys-regulation of microRNAs (miRNAs) may contribute to the pathogenesis of autoimmune diseases, including T1D. This study sought to identify T1D associated miRNAs in the peripheral blood mononuclear cell (PBMC). Peripheral blood mononuclear of newly diagnosed type1 diabetes patients and normal controls were purified by LymphoprepTm gradient purification according to the manufacturer’s instructions (Axis-Shield PoC AS, Oslo, Norway) for futher microarray analysis.

1型糖尿病（Type 1 diabetes mellitus, T1D）是一类由针对胰腺β细胞的自身免疫攻击介导的常见自身免疫性疾病。已有研究表明，微小RNA（microRNAs, miRNAs）的表达失调可能参与包括1型糖尿病在内的多种自身免疫性疾病的发病机制。本研究旨在鉴定外周血单个核细胞（peripheral blood mononuclear cell, PBMC）中与1型糖尿病相关的微小RNA。研究人员依照制造商操作说明，采用挪威奥斯陆Axis-Shield PoC AS公司出品的Lymphoprep™梯度纯化试剂，对新确诊的1型糖尿病患者与正常对照的外周血单个核细胞进行纯化，以用于后续的微阵列分析。