Roles for ATF6-regulated miRNAs in the ER stress response. Mus musculus

We wished to determine the effects of activating the transcription factor, ATF6, on global miRNA expression. We utilized transgenic mice with a conditionally tamoxien-responsive form of ATF6 and assessed cardiac lysates from NTG and TG mice, both treated with tamoxifen and untreated, in order to identify differentially expressed miRNAs. We then focused on miRNAs of interest as well as the genes they are predicted to regulate. Overall design: Four sample groups were assessed for miRNA expression: non-transgenic (NTG) mice treated with vehicle, NTG mice treated with tamoxifen, ATF6 transgenic (TG) mice treated with vehicle, and TG mice treated with tamoxifen

本研究旨在探究激活转录因子ATF6（Activating Transcription Factor 6）对全局miRNA（microRNA）表达的影响。本研究采用携带条件性他莫昔芬应答型ATF6的转基因小鼠，分别收集经他莫昔芬处理及未处理的非转基因（NTG，non-transgenic）与转基因（TG，transgenic）小鼠的心脏裂解物，以鉴定差异表达的miRNA。后续本研究聚焦于目标miRNA及其预测调控的靶基因。实验整体设计：共设置4组样本用于miRNA表达分析：赋形剂处理的非转基因（NTG）小鼠、他莫昔芬处理的非转基因（NTG）小鼠、赋形剂处理的ATF6转基因（TG）小鼠，以及他莫昔芬处理的ATF6转基因（TG）小鼠。